John White (c. 1539 – c. 1593) was an English colonial governor, explorer, artist, and cartographer. White was among those who sailed with Richard Grenville in the first attempt to colonize Roanoke Island in 1585, acting as artist and mapmaker to the expedition. He would most famously briefly serve as the governor of the second attempt to found Roanoke Colony on the same island in 1587 and discover the colonists had mysteriously vanished.

During his time at Roanoke Island he made several watercolor sketches of the surrounding landscape and the native Algonkin peoples. These works are significant as they are the most informative illustrations of a Native American society of the Eastern seaboard; the surviving original watercolors are now preserved in the print room of the British Museum.

In 1587, White became governor of Sir Walter Raleigh's failed attempt at a permanent settlement on Roanoke Island, known to history as the "Lost Colony". This was the earliest effort to establish a permanent English colony in the New World. White's granddaughter Virginia Dare was the first English child born in North America. In late 1587 White returned to England for supplies. The return expedition was delayed due to various reasons, including the Spanish Armada. Governor White finally returned to Roanoke Island in August 1590, but found the colony had been long deserted.

After the failure of the colony, White retired to Raleigh's estates in Ireland, reflecting upon the "evils and unfortunate events" which had ruined his hopes in America, though never giving up hope that his daughter and granddaughter were still alive.