STS-38 was a Space Shuttle mission by NASA using the Space Shuttle Atlantis. It was the 37th shuttle mission, and carried a classified payload for the U.S. Department of Defense (DoD). It was the seventh flight for Atlantis and the seventh flight dedicated to the Department of Defense. The mission was a 4-day mission that traveled 3,291,199 km (2,045,056 mi) and completed 79 revolutions. Atlantis landed at Kennedy Space Center's Shuttle Landing Facility's runway 33. The launch was originally scheduled for July 1990, but was rescheduled due to a hydrogen leak found on Space Shuttle Columbia during the STS-35 countdown. During a rollback to the Orbiter Processing Facility Atlantis was damaged during a hail storm. The eventual launch date of 15 November 1990 was set due to a payload problem. The launch window was between 18:30 and 22:30 EST. The launch occurred at 18:48:13 EST.

The Space Shuttle program was the fourth human spaceflight program carried out by the U.S. National Aeronautics and Space Administration (NASA), which accomplished routine transportation for Earth-to-orbit crew and cargo from 1981 to 2011. Its official name, Space Transportation System (STS), was taken from a 1969 plan for a system of reusable spacecraft of which it was the only item funded for development.The Space Shuttle—composed of an orbiter launched with two reusable solid rocket boosters and a disposable external fuel tank—carried up to eight astronauts and up to 50,000 lb (23,000 kg) of payload into low Earth orbit (LEO). When its mission was complete, the orbiter would reenter the Earth's atmosphere and land like a glider at either the Kennedy Space Center or Edwards Air Force Base.

The Shuttle is the only winged crewed spacecraft to have achieved orbit and landing, and the first reusable crewed space vehicle that has made multiple flights into orbit. Its missions involved carrying large payloads to various orbits including the International Space Station (ISS), providing crew rotation for the space station, and performing service missions on the Hubble Space Telescope. The orbiter also recovered satellites and other payloads (e.g., from the ISS) from orbit and returned them to Earth, though its use in this capacity was rare. Each vehicle was designed with a projected lifespan of 100 launches, or 10 years' operational life. Original selling points on the shuttles were over 150 launches over a 15-year operational span with a 'launch per month' expected at the peak of the program, but extensive delays in the development of the International Space Station never created such a peak demand for frequent flights.